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產品名稱：重組人中腦的Astrocyte-Derived神經營養因子蛋白
產品型號：rHuMANF
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組人中腦的Astrocyte-Derived神經營養因子蛋白與其它公司提供的重組蛋白不同，rHuMANF蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人中腦的Astrocyte-Derived神經營養因子蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人中腦的Astrocyte-Derived神經營養因子蛋白

Synonyms ARMET

Species Human

Accession P55145

GeneID 7873

Source 重組人中腦的Astrocyte-Derived神經營養因子蛋白Escherichia coli.

Molecular Weight Approximately 18.2 kDa, a single non-glycosylated polypeptide chain containing158 amino acids.

Quantity 5μg/25μg/1000μg

AA Sequence LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組人中腦的Astrocyte-Derived神經營養因子蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using rat C6 cells is less than 20 μg/ml, corresponding to a specific activity of > 50 IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Endotoxin Less than 1 EU/μg of rHuMANF as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage 重組人中腦的Astrocyte-Derived神經營養因子蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人中腦的Astrocyte-Derived神經營養因子蛋白

SDS-PAGE

Reference 1. Petrova P, Raibekas A, Pevsner J, et al. 2003. J Mol Neurosci. 20:173-88.
2. Airavaara M, Chiocco MJ, Howard DB, et al. 2010. Exp Neurol. 225:104-13.
3. Lindholm P, Peranen J, Andressoo JO, et al. 2008. Mol Cell Neurosci. 39:356-71.

Background MANF is a secreted neurotrophic factor that is expressed in brain, neuronal and certain non-neuronal tissues. It has been shown to promote survival, growth and function of dopamine specific neurons. MANF and its structural homolog CDNF, each contain an N-terminal saposin-like lipid binding domain, and a carboxyl-terminal domain, which is not homologous to previously characterized protein structures. MANF and CDNF can prevent 6-OHDA induced degeneration of dopaminergic neurons by triggering survival pathways in a rat experimental model of Parkinson disease. Mature human MANF is 99 %, 98 % and 96 % a.a. identical to mature rat, mouse and bovine MANF respectively.

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Synonyms	ARMET
Species	Human
Accession	P55145
GeneID	7873
Source	重組人中腦的Astrocyte-Derived神經營養因子蛋白Escherichia coli.
Molecular Weight	Approximately 18.2 kDa, a single non-glycosylated polypeptide chain containing158 amino acids.
Quantity	5μg/25μg/1000μg
AA Sequence	LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組人中腦的Astrocyte-Derived神經營養因子蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using rat C6 cells is less than 20 μg/ml, corresponding to a specific activity of > 50 IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin	Less than 1 EU/μg of rHuMANF as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	重組人中腦的Astrocyte-Derived神經營養因子蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人中腦的Astrocyte-Derived神經營養因子蛋白
SDS-PAGE
Reference	1. Petrova P, Raibekas A, Pevsner J, et al. 2003. J Mol Neurosci. 20:173-88. 2. Airavaara M, Chiocco MJ, Howard DB, et al. 2010. Exp Neurol. 225:104-13. 3. Lindholm P, Peranen J, Andressoo JO, et al. 2008. Mol Cell Neurosci. 39:356-71.
Background	MANF is a secreted neurotrophic factor that is expressed in brain, neuronal and certain non-neuronal tissues. It has been shown to promote survival, growth and function of dopamine specific neurons. MANF and its structural homolog CDNF, each contain an N-terminal saposin-like lipid binding domain, and a carboxyl-terminal domain, which is not homologous to previously characterized protein structures. MANF and CDNF can prevent 6-OHDA induced degeneration of dopaminergic neurons by triggering survival pathways in a rat experimental model of Parkinson disease. Mature human MANF is 99 %, 98 % and 96 % a.a. identical to mature rat, mouse and bovine MANF respectively.

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產品名稱：重組人中腦的Astrocyte-Derived神經營養因子蛋白