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產品名稱：重組小鼠LPS誘導的CXC趨化因子/ CXCL5蛋白
產品型號：rMuLIX/CXCL5
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組小鼠LPS誘導的CXC趨化因子/ CXCL5蛋白與其它公司提供的重組蛋白不同，rMuLIX/CXCL5蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組小鼠LPS誘導的CXC趨化因子/ CXCL5蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組小鼠LPS誘導的CXC趨化因子/ CXCL5蛋白

Synonyms Cytokine LIX, Small-inducible Cytokine B5

Species Murine

Accession P50228

GeneID 20311

Source 重組小鼠LPS誘導的CXC趨化因子/ CXCL5蛋白Escherichia coli.

Molecular Weight Approximately 9.8 kDa, a single, non-glycosylated polypeptide chain containing 92 amino acids.

Quantity 5μg/20μg/1000μg

AA Sequence APSSVIAATE LRCVCLTVTP KINPKLIANL EVIPAGPQCP TVEVIAKLKN QKEVCLDPEA PVIKKIIQKI LGSDKKKAKR NALAVERTAS VQ

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組小鼠LPS誘導的CXC趨化因子/ CXCL5蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 10-100 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Endotoxin Less than 1 EU/μg of rMuLIX/CXCL5 as determined by LAL method.

Reconstitution 重組小鼠LPS誘導的CXC趨化因子/ CXCL5蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組小鼠LPS誘導的CXC趨化因子/ CXCL5蛋白

SDS-PAGE

Reference 1. Chang MS, McNinch J, Basu R, et al. 1994. J Biol Chem. 269:25277-82.
2. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42.
3. Wuyts A, Haelens A, Proost P, et al. 1996. J Immunol. 157:1736-43.
4. Wuyts A, D'Haese A, Cremers V, et al. 1999. J Immunol. 163:6155-63.

Background CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78) and the gene for CXCL5 is encoded by the CXCL5 gene. It May participate in the recruitment of inflammatory cells by injured or infected tissue. CXCL5 is cleaved into the following 2 chains: GCP-2 (1-78) and GCP-2 (9-78) which attract neutrophils and are involved in neutrophil activation. Recombinant Mouse CXCL5 (92 a.a.) contains 92 amino acids and shares 57 % amino acid sequence identity with human CXCL5.

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Synonyms	Cytokine LIX, Small-inducible Cytokine B5
Species	Murine
Accession	P50228
GeneID	20311
Source	重組小鼠LPS誘導的CXC趨化因子/ CXCL5蛋白Escherichia coli.
Molecular Weight	Approximately 9.8 kDa, a single, non-glycosylated polypeptide chain containing 92 amino acids.
Quantity	5μg/20μg/1000μg
AA Sequence	APSSVIAATE LRCVCLTVTP KINPKLIANL EVIPAGPQCP TVEVIAKLKN QKEVCLDPEA PVIKKIIQKI LGSDKKKAKR NALAVERTAS VQ
Purity	> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組小鼠LPS誘導的CXC趨化因子/ CXCL5蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 10-100 ng/ml.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Endotoxin	Less than 1 EU/μg of rMuLIX/CXCL5 as determined by LAL method.
Reconstitution	重組小鼠LPS誘導的CXC趨化因子/ CXCL5蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組小鼠LPS誘導的CXC趨化因子/ CXCL5蛋白
SDS-PAGE
Reference	1. Chang MS, McNinch J, Basu R, et al. 1994. J Biol Chem. 269:25277-82. 2. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42. 3. Wuyts A, Haelens A, Proost P, et al. 1996. J Immunol. 157:1736-43. 4. Wuyts A, D'Haese A, Cremers V, et al. 1999. J Immunol. 163:6155-63.
Background	CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78) and the gene for CXCL5 is encoded by the CXCL5 gene. It May participate in the recruitment of inflammatory cells by injured or infected tissue. CXCL5 is cleaved into the following 2 chains: GCP-2 (1-78) and GCP-2 (9-78) which attract neutrophils and are involved in neutrophil activation. Recombinant Mouse CXCL5 (92 a.a.) contains 92 amino acids and shares 57 % amino acid sequence identity with human CXCL5.

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產品名稱：重組小鼠LPS誘導的CXC趨化因子/ CXCL5蛋白